不同日龄犊牛睾丸中细胞周期调控基因CyclinB1和p34cdc2表达的差异

doi:10.11843/j.issn.0366-6964.2015.02.010

Abstract

Abstract:

This study was performed to investigate the expression of cell cycle regulation genes CyclinB1 and p34^cdc2 in testis of calves at different ages.The eighteen 5-6 days healthy Holstein calves (with similar weight) were randomly divided into 3 groups and fed with the similar basal diet.The testes of 2 calves from each group were collected at the time of 30 ，60 and 90 d respectively.QRT-PCR method was used to detect the mRNA expression of the cell cycle regulatory genes CyclinB1 and p34^cdc2 in testes of calves at different ages.The location of CyclinB1 and p34^cdc2 in the testes was detected by immunohistochemical methods.The results showed that the highest expression of CyclinB1 and p34^cdc2 mRNA and protein was obtained in the calves’ testes of 90 d.The mRNA and protein expression levels of CyclinB1 and p34^cdc2 increased with the increasing age.The mRNA expression level of the 2 genes at the age of 90 d were significantly higher than that at 30 and 60 d (P<0.05).However，no significant difference was observed between 30 and 60 d (P>0.05).The protein expression of p34^cdc2 in the testes of calves at different ages was significant (P<0.05).Although the protein expression of CyclinB1 at 60 and 90 d had no significant difference(P>0.05)，they were significantly higher than those at 30 d.This study indicated that the expression of CyclinB1 and p34^cdc2 mRNA and protein increased with the increasing age and CyclinB1 and p34^cdc2 in the testes of calves at different ages was significant.

CLC Number:

S823.2

YAO Xiao-lei，SONG Rui-gao，Lü Li-hua，LI Peng-fei，LIU Qiang，HUANG Yang，CHEN Jian-wei，JIANG Xiao-long，CAO Xia，ZHAO Miao-miao，ZHANG Gui-hua，WANG Yong-xin，SHI Lei. Expressions of Cell Cycle Regulation Genes CyclinB1 and p34^cdc2 in the Testes of Calves Aged on Different Days[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(2): 245-250.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2015.02.010

https://www.xmsyxb.com/EN/Y2015/V46/I2/245

References

［1］DOREE M，HUNT T.From Cdc2 to Cdk1：when did the cell cycle kinase join its partner ［J］.J Cell Sci，2002，115:2461-2464.
［2］HARTWELL L H，WEINERT T A.Checkpoints：Controls that ensure the order of cell cycle events ［J］.Science，1989，246:629-634.
［3］KAUSHAL N，BANSAL M P.Inhibition of Cdc2/CyclinB1 in response to selenium-induced oxidative stress during spermatogenesis：potential role of Cdc25c and p21 ［J］.Mol Cell Biochem，2007，298(1-2):139-50.
［4］BLETHROW J D，GLAVY J S，MORGAN D O，et al.Covalent capture of kinase-specific phosphopeptides reveals Cdk1-cyclin B substrates ［J］.Proc Natl Acad Sci, 2008，105：1442-1447.
［5］CALLARD G V，JORGENSEN J C，REDDING J M.Biochemical analysis of programmed cell death during premeiotic stages of spermatogenesis in vivo and in vitro ［J］.Dev Genet, 1995，162:1401.
［6］SINHA-HIKIMI A P，LUE Y，DIAZ-ROMERO M，et al. Deciphering the pathways of germ cell apoptosis in the testis ［J］.J Steroid Biochem Mol Biol，2003,85(2)：175-182.
［7］SINHA HIKIM A P，SWERDLOFF R S.Hormonal and genetic control of germ cell apoptosis in the testis ［J］. Rev Reprod，1999，4:38-47.
［8］YUAN J,KRAMER A,MATTHESS Y,et al.Stable gene silencing of CyclinB1 in tumor cells increases susceptibility to taxol and leads to growth arrest in vivo ［J］.Oncogene，2006，25(12):1753-1762.
［9］KONG W H,ZHENG G U,JI NING L U,et al.Temperature dependent expression of p34^cdc2 and CyclinB1 in spermatogenic cells during spermatogenesis ［J］.Cell，2000，10：289-302.
［10］YU Q,WU J.Involvement of cyclins in mammalian spermatogenesis ［J］.Mol Cell Biochem, 2008，315(1-2):17-24.
［11］POOLMAN R A，BROOKS G.Expressions and activities of cell cycle regulatory molecules during the transition from myocyte hyperplasia to hypertrophy ［J］.J Mol Cell Cardiol,1998，30(10):2121-2135.
［12］CHARRIER-SAVOURNIN F B,CHATEAU M T,GIRE V,et al.P21-mediated nuclear retention of CyclinB1-CDK1 in response to genotoxic stress ［J］.Mol Biol Cell,2004,15：3965-3976.
［13］YU F,YANG G,ZHAO Z,et al.Apoptosis related protein 3，an ATRA-up regulated membrane protein arrests the cell cycle at G1/S phase by decreasing the expression of CyclinD1［J］.Biochem Biophys Res Commun,2007,358:1041-1046.
［14］YU B Z，SONG Y T，YU D H，et al.Expression and immunohistochemical localization of Cdc2 and P70S6K in different stages of mouse germ cells ［J］.Cell Biochem Funct,2006，24(2):113-117.
［15］QIU G F,RAMACHANDRA R K,REXROAD C E，et al.Molecular characterization and expression profiles of CyclinB1，B2 and Cdc2 kinase during oogenesis and spermatogenesis in rainbow trout ［J］.Anim Reprod Sci，2008，105(3)：209-225.
［16］朱士恩.动物生殖生理［M］.北京:中国农业出版社，2005.
ZHU S E.Animal reproductive physiology ［M］.Beijing：China Agriculture Press，2005.(in Chinese)
［17］OSHAUGHNESSY P J，BAKER P J，JOHNSTON H.The foetal leyding cell differentiation，function and regulation ［J］.Int J Androl，2006，29(1)：90-95.
［18］位兰,彭克美,罗来强.黄体生成素和褪黑素受体在不同日龄雏鸵鸟睾丸内的分布研究［J］.经济动物学报,2011,15(1):25-28.
WEI L，PENG K M，LUO L Q.Distribution of luteinizing hormone and melatonin receptor in the testis during developmental period of Ostrich chicks ［J］.Journal of Economic Animal，2011，15(1):25-28.(in Chinese)
［19］REN Y，WANG Q，SHI L，et al.Effects of maternal and dietary selenium (Se-enriched yeast) on the expression of p34^Cdc2 and CyclinB1 of germ cells of their offspring in goats［J］.Anim Reprod Sci，2011，123:187-191.
［20］HAN K，DAI Y，ZOU Z，et al.Molecular characterization and expression profiles of Cdc2 and CyclinB1 during oogenesis and spermatogenesis in green mud crab ［J］.Comp Biochem Physiol Biochem Mol Biol，2012，(163):292-302.
［21］王锦荣,杨章平,臧胜兵,等.荷斯坦奶牛生长发育规律研究［J］.安徽农业科学，2013,41(8):3426-3428.
WANG J R，YANG Z P，ZANG S B，et al.Growth and development law of Holstein cows ［J］.Journal of Anhui Agricultural Sciences，2013,41(8)：3426-3428.(in Chinese)
［22］郑丕留，李国贤,蒋世娥,等.公猪2~8月龄生殖器官发育的解剖学及组织学观察［J］.畜牧兽医学报，1965，8(4):233-246.
ZHENG P L，LI G X，JIANG S E，et al.Some anatomical and histological studies on the development of reproductive organs of 2 to 8 mouth-old boars ［J］.Acta Veterinaria et Zootechnica Sinica，1965，8(4):233-246.(in Chinese)

[1]	MIAO Shu, AN Jishan, WANG Zuo, XIAO Dingfu, LAN Xinyi, LIU Lei, SHEN Weijun, WAN Fachun. Leucine Promotes the Proliferation of Bovine Myoblasts through PI3K-AKT Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 142-152.
[2]	NIU Yifan, YANG Baigao, ZHANG Peipei, ZHANG Hang, FENG Xiaoyi, CAO Jianhua, YU Zhou, HAO Haisheng, DU Weihua, ZOU Huiying, ZHU Huabin, MA Youji, ZHAO Xueming. Advances in Bovine Embryo Genome Selection [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4449-4457.
[3]	WANG Wenxiang, DU Lili, HU Junwei, ZHANG Yanxiang, MA Minghao, DUAN Rui, QIAN Cong, WANG Xinyue, LI Sanlu, ZHANG Changqing, ZHANG Lupei, GAO Xue, XU Lingyang, LI Junya, GAO Huijiang. Mining Candidate Genes Related to Meat Quality Traits of Longissimus Lumborum in Pingliang Red Steer Based on Transcriptome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4589-4604.
[4]	MA Haoran, ZHANG Lupei, JIN Shengyun, BAO Jinshan, LI Hongyan, GAO Huijiang, XU Lingyang, WANG Zezhao, LI Junya. Assessment of the Genomic Relationships for Chinese Indigenous Beef Cattle Using High-density SNP Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4174-4185.
[5]	ZHANG Junxing, ZHANG Hailiang, HAN Liyun, MA Yanfen, WEN Wan, ZHOU Jiamin, TIAN Jia, LU Tingting, MA Yun, WANG Yachun. Analysis on the Influencing Factors of Wellness Traits in Holstein Lactating Cows in Ningxia [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2389-2401.
[6]	YANG Chuang, WU Longfei, LIU Guangbin, LI Yaokun, LIU Dewu, SUN Baoli. Expression Profile and Bioinformatics Analysis of lncRNA and Its Associated ceRNA Networks in Longissimus Dorsi from Lufeng Cattle and Leiqiong Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1951-1963.
[7]	GUO Mingpeng, MENG Yuan, WANG Honghao, WANG Yuan, CHE Leijie, SHEN Li, WANG Xi. Estimation of Genetic Parameters of Body Weight and Body Size Traits in Jinnan Cattle at Different Growth Stages [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1452-1464.
[8]	YU Shiqiang, LI Liuxue, ZHAO Xiaobo, ZHAO Huiying, TU Yan, ZHAO Yuchao, JIANG Linshu. Differences and Correlations of Lactation Performance in Chinese Holstein Dairy Cows at Different Lactation Stages and Somatic Levels [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1003-1014.
[9]	YANG Guang, XU Jing, LI Xin, HU Debao, GUO Yiwen, DING Xiangbin, GUO Hong, ZHANG Linlin. Effect of Interfering lncbMD on Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1015-1025.
[10]	MA Zhijie, WANG Shikang, ZHANG Weizhong, GUO Weixing, ZHAO Haiming, LEI Chuzhao. Y-chromosome Genomic Diversity and Paternal Origins of Qaidam Cattle and Mongolian Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1026-1033.
[11]	NIU Ruili, SONG Hanan, WU Yue, WANG Yunan, GAO Yefan, GUAN Weijun, ZONG Xianchun. Isolation, Culture and Biological Characteristics of Epidermal Stem Cells from Simmental Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 168-177.
[12]	LI Hongwei, XU Lingyang, WANG Zezhao, CAI Wentao, ZHU Bo, CHEN Yan, GAO Xue, ZHANG Lupei, GAO Huijiang, LI Junya. Genome-wide Association Study of Slaughter Traits Based on Haplotype in Beef Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4232-4243.
[13]	CHANG Yao, SU Guosheng, LI Yanhua, LI Xiang, MA Zhu, WANG Yachun. Estimating Genetic Parameters for Body Weights using Pedigree and Genotype-pedigree based Approaches in Holstein Heifers [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3759-3768.
[14]	XU Jingyi, XU Haoqi, HU Lirong, ZHANG Fan, GAO Qing, LUO Hanpeng, ZHANG Hailiang, SHI Rui, LI Xiang, LIU Lin, GUO Gang, WANG Yachun. Association Analysis of MET Gene Single Nucleotide Polymorphism with Reproduction and Milk Production Traits in Chinese Holstein Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3769-3785.
[15]	WANG Chuanchuan, MU Tong, FENG Xiaofang, YU Baojun, ZHANG Juan, GU Yaling. Identification of Key Candidate Genes for Milk Fat Metabolism in Dairy Cows Based on Transcriptome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3421-3433.

Expressions of Cell Cycle Regulation Genes CyclinB1 and p34^cdc2 in the Testes of Calves Aged on Different Days

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

Expressions of Cell Cycle Regulation Genes CyclinB1 and p34cdc2 in the Testes of Calves Aged on Different Days

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

Expressions of Cell Cycle Regulation Genes CyclinB1 and p34^cdc2 in the Testes of Calves Aged on Different Days